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为探讨黄芪注射液治疗小鼠柯萨奇R_3(CVB_3)病毒性心肌炎的疗效及其机制,将Balb/C鼠24只腹腔感染CVB_3亲心肌细胞株后,随机分为两组:黄芪治疗组(黄芪注射液10g/ks·d腹腔注射)和对照组。实验第8天留取心肌标本,进行心肌病理检查和心肌穿孔素mRNA逆转录多聚酶链反应(RT-PCR)分析。结果显示:①黄芪治疗组心肌病变积分明显低于对照组(1.70±0.51比2.98±0.54,P<0.01);②黄芪治疗组心肌穿孔素mRNA的表达明显低于对照组(1.10±0.07比1.31±0.12,P<0.01);③心肌穿孔素mRNA表达水平与心肌病变积分呈显著正相关(r=0.88,P<0.01)。结论:穿孔素介导的细胞毒性作用可能在病毒性心肌炎的发病中起重要作用。黄芪注射液可能具有抗病毒、调节免疫和减轻心肌损伤的作用。
To investigate the efficacy and mechanism of Huangqi injection in treating Coxsackie R_3 (CVB_3) viral myocarditis in mice, 24 Balb/C mice were infected intraperitoneally with CVB_3 myocardiomyocytes and randomly divided into two groups: Astragalus treatment group ( Astragalus Injection 10g/ks·d intraperitoneally) and control group. On the 8th day of the experiment, myocardial specimens were taken for myocardial pathological examination and myocardial perforin mRNA reverse transcription polymerase chain reaction (RT-PCR) analysis. The results showed that: 1 The score of myocardial lesions in the treatment group was significantly lower than that in the control group (1.70±0.51 vs. 2.98±0.54, P<0.01); 2 The mRNA expression of perforin in the treatment group was significantly lower than that in the control group (1.10±0.07 vs 1.31) (±0.12, P<0.01); 3 There was a significant positive correlation between myocardial perforin mRNA expression and myocardial lesion scores (r=0.88, P<0.01). CONCLUSION: Perforin-mediated cytotoxicity may play an important role in the pathogenesis of viral myocarditis. Astragalus injection may have antiviral effect, regulate immunity and reduce myocardial damage.