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目的观察同位素标记的抗表皮生长因子单克隆抗体ＥＱ７５对胶质瘤细胞的亲和力及杀伤力。方法体外实验：采用荧光显微镜及流式细胞仪荧光强度定量分析进行ＥＱ７５对人胶质瘤细胞株ＢＴ３２５表面抗原特异性鉴定。体内试验：建立裸小鼠移植性胶质瘤模型，随机分组，经尾静脉分别注入１１１Ｉｎ┐ＥＱ７５和１１１Ｉｎ┐ＩｇＧ，４８ｈ后测定各脏器组织Ｔ／ＮＴ放射性比值，连续２周观测肿瘤移植物的大小，比较荷瘤小鼠的生存时间。结果体外试验：ＥＱ７５对ＢＴ３２５有高度的亲和性。体内试验：ＥＱ７５组Ｔ／ＮＴ、肿瘤移植物的抑制率以及荷瘤小鼠的生存时间均高于对照组（Ｐ＜０．０１）。结论１１１Ｉｎ┐ＥＱ７５对胶质瘤细胞有良好的亲和力及杀伤力。
Objective To observe the affinity and lethality of isotope-labeled anti-EGF monoclonal antibody EQ75 on glioma cells. Methods: In vitro experiments: The surface antigen specificity of human glioma cell line BT325 was identified by fluorescence microscopy and fluorescence intensity analysis by flow cytometry. In vivo: A nude mouse model of transplanted glioma was established and randomly divided into groups. 111 In. EQ75 and 111 In. IgG were injected through the tail vein. After 48 hours, the T/NT radioactivity ratio of each organ was measured, and the tumor grafts were observed for 2 consecutive weeks. The size of the tumor-bearing mice was compared with their survival time. Results In vitro test: EQ75 has a high affinity for BT325. In vivo tests: The inhibition rate of T/NT, tumor grafts, and tumor-bearing mice in the EQ75 group were higher than those in the control group (P<0.01). Conclusion 111IndEQ75 has good affinity and killing ability on glioma cells.