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苗高是水稻生长发育的重要指标,碱胁迫会使水稻苗高明显降低。本研究以碱敏感的粳稻品种彩稻为母本与强耐碱的籼稻品种WD20342为父本杂交衍生的F2:3群体为初定位作图群体,构建了包含151个SSR标记的遗传连锁图谱;在0.15%Na2CO3胁迫条件下,对水稻苗期的苗高进行QTL初步定位。共检测到3个与碱胁迫下苗高相关的QTL,分布在第5、7和11染色体上,其中q ASH-7的贡献率最大,为20.09%,是一个主效QTL。以彩稻为轮回亲本进行分子标记辅助选择回交,构建了BC2F2:3次级定位群体,在初定位区间进行SSR标记加密,将q ASH-7定位在第7染色体RM336~RM5495遗传距离0.9 c M、物理距离180.67 kb的区域内,共预测出23个编码基因。本研究结果为水稻苗期苗高QTL更加精细的定位以及图位克隆提供了研究基础。
Seedling height is an important indicator of rice growth and development, alkali stress will significantly reduce the rice seedling height. In this study, F2: 3 F2: 3 populations derived from a cross between an indica rice variety resistant to alkali-sensitive japonica rice variety WD20342 and its parental susceptible japonica rice were used as initial loci to construct a genetic linkage map containing 151 SSR markers. Under 0.15% Na2CO3 stress, the QTL for seedling height at rice seedling stage was initially mapped. Three QTLs related to seedling height under alkali stress were detected on chromosomes 5, 7 and 11, of which QASH-7 contributed the most to 20.09%, which is a major QTL. The second round of BC2F2: 3 loci was constructed by marker-assisted selection backcrossing of the reciprocal hybrid rice. The SSR marker was used to encrypt the loci in the initial locus, and the genetic distance of q ASH-7 was located on the chromosome 7 RM336 ~ RM5495 by 0.9 c M, the physical distance of 180.67 kb region, a total of 23 coding genes predicted. The results of this study provide the basis for more precise mapping and cloning of QTL for seedling height in rice.