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目的观察去势骨质疏松模型大鼠在铁过载状况下骨转换和骨密度变化,探讨铁过载与绝经后骨质疏松症的相关作用。方法将32只3月龄雌性SD大鼠按完全随机法平分为假手术组(Sham组)、去势组(OVX组)、去势基础上2种剂量枸橼酸铁铵组(FAC)干预组,即低剂量枸橼酸铁铵干预组(FAC1组)、高剂量枸橼酸铁按干预组(FAC2组)。大鼠行双侧卵巢切除术,去势后1周分别用枸橼酸铁铵90(FAC1)和180 mg/kg(FAC2)腹腔注射,每周2次,共9周,OVX组和Sham组按同样方式和频次注射等量生理盐水。9周后心脏取血,检测血清铁、β-Ⅰ型胶原羧基端肽(β-CTX)、骨钙素(BGP)含量。用肝脏Perl’s法铁染色,用原子吸收法检测胫骨铁含量,观察股骨病理改变,并对股骨远端Micro-CT行三维分析。结果与Sham组比较,OVX组去势后血清铁含量、肝脏普鲁士蓝铁染色差异无统计学意义,但胫骨铁含量显著下降;与Sham组、OVX组比较,FAC1组、FAC2组血清铁、胫骨铁含量升高,肝脏蓝染铁颗粒显著增加,差异有统计学意义(P<0.05)。与Sham组比较,OVX组β-CTX、BGP高于Sham组(P<0.05),FAC1和FAC2组较OVX组β-CTX增高(P<0.05);但BGP水平在3组间差异无统计学意义。病理组织形态学结果显示,与OVX组比较,铁干预组骨小梁形态结构稀疏,间距增大。Micro-CT分析表明,与OVX组比较,去势后铁干预组骨密度(BMD)、骨小梁厚度(Tb.th)、骨体积分数(BV/TV)、骨小梁数量(Tb.N)均显著下降(均P<0.05),骨小梁分离度(Tb.Sp)、结构模型指数(SMI)、各向异性度(DA)显著升高(均P<0.05)。结论大鼠去势后合并铁过载促进骨吸收,使骨小梁结构进一步稀疏,骨质密度进一步降低。
Objective To observe the changes of bone turnover and bone mineral density in osteoporosis model rats under iron overload and to explore the correlation between iron overload and postmenopausal osteoporosis. Methods Thirty-two 3-month-old female Sprague-Dawley rats were equally randomized to sham operation (Sham) and castration (OVX), and were treated with two doses of ammonium ferric citrate (FAC) Group, namely low-dose iron ammonium citrate intervention group (FAC1 group), high-dose iron citrate intervention group (FAC2 group). Rats were subjected to bilateral ovariectomy. FAC1 and 180 mg / kg (FAC2) were intraperitoneally injected twice a week for 9 weeks in OVX group and Sham group The same way and frequency of injection of the same amount of saline. After 9 weeks, blood was taken from the heart to detect serum iron, β-CTX and BGP contents. Perl’s method was used to stain the liver, and the iron content of the tibia was detected by atomic absorption spectroscopy. The pathological changes of the femur were observed. The distal femur was analyzed by Micro-CT three-dimensional analysis. Results Compared with Sham group, serum iron content and Prussian blue iron staining in OVX group were not statistically significant, but the content of iron in tibia was significantly decreased. Compared with Sham group and OVX group, serum iron and tibia in FAC1 group and FAC2 group Iron content increased, the liver blue stained iron particles increased significantly, the difference was statistically significant (P <0.05). Compared with Sham group, β-CTX and BGP in OVX group were higher than those in Sham group (P <0.05), while those in FAC1 and FAC2 group were higher than those in OVX group (P <0.05); however, there was no significant difference in BGP level between the three groups significance. Histopathological results showed that compared with OVX group, the trabecular bone morphology of spironolactone group was sparse and the spacing increased. Micro-CT analysis showed that compared with OVX group, BMD, Tb.th, BV / TV, and trabecular number (Tb.N) ) (P <0.05). The Tb.Sp, SMI and DA were significantly increased (all P <0.05). Conclusion Overexpression of iron in rats after castration promotes bone resorption, resulting in further sparse trabecular structure and further reduction of bone density.