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目的:研究灰树花胞外多糖(GLP-1)对α-葡萄糖苷酶的抑制作用。方法:发酵培养灰树花,发酵液经醇沉提取得到胞外多糖粗品;经脱蛋白、脱色、流水透析处理得到GLP;组分分离后收集主峰冻干得GLP-1组分。在标准体系下考察GLP-1对α-葡萄糖苷酶的抑制作用。结果:标准体系下测得GLP-1对α-葡萄糖苷酶的半抑制浓度(IC50)为35.79mg·mL-1;GLP-1具有对温度和pH的稳定性;GLP-1对α-葡萄糖苷酶的作用时间4min达到最大;Ki值为3.4×10-4mol.L-1;双倒数曲线表明GLP-1与α-葡萄糖苷酶的作用机制为非竞争性抑制。结论:GLP-1对α-葡萄糖苷酶具有较高的亲和性,可以有效抑制α-葡萄糖苷酶的活性,证实其具有抑制糖吸收的功能。
Objective: To study the inhibitory effect of Grifola frondosa extracellular polysaccharide (GLP-1) on α-glucosidase. Methods: Grifola frondosa was fermented and cultured, and the crude extract of extracellular polysaccharide was obtained by alcohol precipitation. The GLP was obtained by deproteinization, decolorization and flow-through dialysis. The inhibitory effect of GLP-1 on α-glucosidase was investigated in a standard system. Results: The half-inhibitory concentration of GLP-1 to α-glucosidase (IC50) was 35.79 mg · mL-1 under standard system; GLP-1 had stability to temperature and pH; The effect of glycosidase reached the maximum at 4 min. The Ki value was 3.4 × 10 -4 mol·L -1. The double reciprocal curve indicated that the mechanism of action of GLP-1 and α-glucosidase was noncompetitive. CONCLUSION: GLP-1 has high affinity for α-glucosidase and can effectively inhibit the activity of α-glucosidase, confirming that it has the function of inhibiting sugar absorption.