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用多聚酶链反应（ＰＣＲ）扩增Ｅｐｓｔｉｎ－Ｂａｒ病毒（ＥＢＶ）的ＢａｍＨＩＷ片段，在２０例低分化鼻咽癌活检组织中，检测出ＥＢＶＤＮＡ片段；裸鼠低分化ＮＰＣ移植瘤株ＳＵＮＴ－１从第１至第３４代持续存在ＥＢＶＤＮＡ，其相应体外细胞株ＳＵＮＥ－１第１至第６２代仍阳性。用原位溶细胞电泳技术（Ｉｎｓｉｔｕｌｙｓｉｎｇｇｅｌｅｌｅｃｔｒｏｐｈｏｒｅｓｉｓ）结合ＢａｍＨＩＷ片段为探针，进行ＤＮＡ杂交，发现ＳＵＮＥ－１第２０代细胞中有ＥＢＶ线性ＤＮＡ存在。２０例ＮＰＣ活检组织的ＤＮＡ经ＢａｍＨＩ酶切，以ＬＭＰ２Ａ外显子ｅ１～ｅ５为探针，Ｓｏｕｔｈｅｒｎ杂交，１９例均只出现一条带，证明病毒ＤＮＡ不是以线状或整合状，而是以环状附加体形式存在。说明ＥＢＶ感染鼻咽上皮细胞已经过克隆选择，或者其中一个病毒感染的克隆已成为选择优势，提示ＥＢＶ感染在ＮＰＣ癌变过程中起重要作用。
The BamHIW fragment of Epstin-Bar virus (EBV) was amplified by polymerase chain reaction (PCR) and the EBV DNA fragment was detected in 20 cases of poorly differentiated nasopharyngeal carcinoma. The nude mice differentiated NPC tumor cell line SUNT- EBV DNA was persisted from generation 1 to passage 34 and remained positive from generation 1 to generation 62 of the corresponding in vitro cell line SUNE-1. In situ hybridization and BamHIW fragments were used as probes to detect EBV linear DNA in SUNE-1 cells. DNA from 20 cases of NPC biopsies was digested with BamHI, and with LMP2A exons e1 ~ e5 as probe, Southern hybridization showed only one band in 19 cases, which showed that the viral DNA was not linear or integrated, Appendages exist in the form of. EBV infected nasopharyngeal epithelial cells have been clonal selection, or one of the virus-infected clones has become an advantage of choice, suggesting that EBV infection plays an important role in the carcinogenesis of NPC.