目的和方法：取新生ＳＤ大鼠心室肌制备培养搏动心肌细胞，采用放射性同位素４５Ｃａ２＋及３Ｈ－牛磺酸示踪技术，观察钙离子与牛磺酸跨心肌细胞膜内流的相互影响。结果：（１）牛磺酸内流在低胞外Ｃａ２＋浓度（［Ｃａ２＋］ｏ，０．５ｍｍｏｌ／Ｌ）和高［Ｃａ２＋］ｏ（４０ｍｍｏｌ／Ｌ）均比正常［Ｃａ２＋］ｏ（１５ｍｍｏｌ／Ｌ）和高［Ｃａ２＋］ｏ（４０ｍｍｏｌ／Ｌ）时增加，尤以低［Ｃａ２＋］。时明显；异搏定（１０μｍｏｌ／Ｌ）能促进牛磺酸的内流；（２）１０～２０ｍｍｏｌ／Ｌ牛磺酸对不同［Ｃａ２＋］。（０５、１５和４０ｍｍｏｌ／Ｌ）下的Ｃａ２＋内流均有抑制作用，而１ｍｍｏｌ／Ｌ牛磺酸只抑制高［Ｃａ２＋］ｏ时Ｃａ２＋内流，对低［Ｃａ２＋］ｏ和正常［Ｃａ２＋］ｏ时Ｃａ２＋内流无明显作用；１～２０ｍｍｏｌ／Ｌβ－丙氨酸对Ｃａ２＋内流无明显影响。结论：胞外Ｃａ２＋浓度的变化可直接影响牛磺酸内流，而牛磺酸能抑制Ｃａ２＋的内流，两者相互影响，从而发挥牛碘酸调节细胞内Ｃａ２＋浓度的作用 PURPOSE AND METHODS: Pulmonary cardiomyocytes were isolated from neonatal SD rats and the interaction between calcium ions and taurine intramyocardial membrane flux was observed by radioactive isotope 45Ca2 + and 3H-taurine tracer techniques. Results: (1) The influx of taurine was lower than that of normal [Ca2 +] o (1) in low extracellular Ca2 + concentration (0.5 mmol / L) and high [Ca2 +] o ⑤ 5mmol / L) and high [Ca2 +] o (40mmol / L) increased, especially low [Ca2 +]. (10μmol / L) can promote the influx of taurine; (2) 10 ~ 20mmol / L taurine to different [Ca2 +]. (05,  15 and 40mmol / L), while 1mmol / L taurine only inhibited Ca2 + influx in high [Ca2 +] o, There was no significant effect of Ca2 + influx in normal [Ca2 +] o; 1 ~ 20mmol / L β-alanine had no significant effect on Ca2 + influx. CONCLUSION: The change of extracellular Ca2 + concentration can directly affect the influx of taurine, while taurine can inhibit the inward influx of Ca2 +, both of them interact with each other, so that the effect of intracellular Ca2 +