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拟利用非对称原生质体融合技术向芸薹属花椰菜中转移野生抗逆性状。供体Brassica spinescens具有光合效率高,抗白锈病、蚜虫、黑斑病,耐盐等优良特性。用经UV处理的供体叶肉原生质体与花椰菜下胚轴原生质体通过聚乙二醇(PEG)融合,培养后获得379株再生植株。对其中的120株进行过氧化物同工酶分析和RAPD分子标记检测,证明有23株为杂种植株。流式细胞仪倍性分析表明,杂种植株核DNA含量全部高于供、受体亲本核DNA含量总和,植株倍性在四至八倍之间。还对供体不同UV射线处理的剂量进行了初步探索,结果表明0.0750 J.cm-2为最大辐射剂量。
It is proposed to transfer the wild stress resistance traits to Brassica oleracea by asymmetric protoplast fusion technology. The donor Brassica spinescens has excellent photosynthetic efficiency, anti-white rust, aphids, black spot disease, salt tolerance and other good characteristics. 379 regenerated plants were obtained by fusion of polyethylene glycol (PEG) with UV-treated donor mesophyll protoplasts and cauliflower hypocotyl protoplasts. Peroxidase isoenzyme analysis and RAPD molecular marker analysis of 120 of them showed that 23 were hybrid plants. Ploidy analysis by flow cytometry showed that the nuclear DNA content of hybrid plants were all higher than the sum of the DNA content of donor and recipient parent nuclei, and the plant ploidy was between four and eight times. The dosages of different UV-irradiated donors were also explored. The results showed that 0.0750 J.cm-2 was the maximum radiation dose.