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探讨体外培养条件下肿瘤组织和原代培养肿瘤细胞对鸡胚神经节神经元的影响。方法 :⑴贲门癌组织块和原代培养的肿瘤细胞与鸡胚背根神经节共培养 ;⑵贲门癌组织 /原代细胞培养液与鸡胚背根神经节和交感神经节共培养 ;⑶贲门癌组织 /原代细胞培养液中加入神经生长因子 (nervegrowthfactor ,NGF)抗体后与鸡胚背根神经节和交感神经节共培养。 结果 :⑴贲门癌组织块及原代培养的肿瘤细胞对神经节突起有促生长和诱向作用 ;⑵肿瘤组织 /原代肿瘤细胞培养液也能促使背根神经节和交感神经节长出浓密突起 ;⑶NGF抗体只能部分阻断这种促神经生长作用。结论 :体外培养的贲门癌组织和细胞对神经节突起有促生长和诱向作用 ,这种作用可能部分与神经生长因子有关。
To investigate the effect of tumor tissue and primary cultured tumor cells on chick embryo ganglion neurons under in vitro culture conditions. Methods: (1) Cardiac cancer tissue blocks and primary cultured tumor cells were co-cultured with chick embryo dorsal root ganglia; (2) Cardiac cancer tissue/primary cell cultures were co-cultured with chick embryo dorsal root ganglia and sympathetic ganglia; (3) The nerve growth factor (NGF) antibody was added to the cancer tissue/primary cell culture medium and co-cultured with the chick embryo dorsal root ganglion and sympathetic ganglion. Results: (1) Cardiac cancer tissue blocks and primary cultured tumor cells promote growth and induce neurite prominence; (2) Tumor tissue/primary tumor cell culture media also promoted the growth of dorsal root ganglia and sympathetic ganglia. Protrusion; (3) NGF antibody can only partially block this nerve growth promoting effect. Conclusion : In vitro cultured cardiac cancer tissues and cells can promote growth and induce ganglion processes. This effect may be partly related to nerve growth factor.