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目的通过观察益气活血中药补阳还五汤对脑出血大鼠血肿周围新生血管内皮整合素αVβ3和整合素β3亚基表达的影响,探讨益气活血法治疗脑出血的作用机制。方法 150只SD大鼠随机分为假手术组、模型组、益气组、活血组、益气活血组(补阳还五汤),通过立体定位向脑内苍白球注入Ⅶ型胶原酶0.5 U建立脑出血模型。假手术和模型组灌服蒸馏水,余各组灌服补阳还五汤及其益气组分、活血组分药物。运用免疫组织化学方法,并对结果进行半定量分析,检测各组术后3、7、14、21、28 d各时点血肿周围新生血管内皮整合素αVβ3和整合素β3亚基的时空表达。结果除假手术组,各组新生血管内皮整合素αVβ3和β3亚基的表达趋势大致相近,7 d时各组表达达到高峰,益气活血组高峰延长至14 d,且各时点的表达较模型组增高(P<0.05)。结论补阳还五汤通过益气活血作用,能调节脑出血大鼠血肿周围新生血管整合素αVβ3和β3亚基的表达;通过参与脑出血后的血管新生,影响微血管系统重建,从而达到修复损伤组织的目的 。
Objective To observe the effect of Buyang Huanwu Decoction (TCM Formula for Invigorating Qi and Promoting Blood Circulation) on the expression of integrin αVβ3 and integrin β3 subunit of new blood vessels around the hematoma in rats with intracerebral hemorrhage and to explore the mechanism of Yiqi Huoxue Decoction in treating cerebral hemorrhage. Methods 150 Sprague-Dawley rats were randomly divided into sham-operation group, model group, Yiqi group, Huoxue group and Yiqi Huoxue group (BYHWD Decoction). 0.5 U Establishment of intracerebral hemorrhage model. Sham operation and model group were fed with distilled water. The remaining groups were fed with BYHWD and its Qi, Huoxu component medicines. Immunohistochemistry and semi-quantitative analysis of the results were performed to detect the spatial and temporal expression of integrin αVβ3 and integrin β3 subunits in neovascular tissue around the hematoma at 3, 7, 14, 21 and 28 d after operation. Results The expression of integrin αVβ3 and β3 subunit of neovascular endothelial cells in each group was similar to that of the sham - operation group. The expression reached its peak at 7 days, and the peak of Yiqi Huoxue group was prolonged to 14 days. Model group increased (P <0.05). Conclusion Buyang Huanwu Decoction can regulate the expression of integrin αVβ3 and β3 subunits of neovascularization around the hematoma in rats by means of invigorating qi and activating blood circulation. It can repair angiogenesis by affecting the angiogenesis after intracerebral hemorrhage and thus rehabilitate the microvascular system The purpose of the organization.