论文部分内容阅读
目的建立一种测定滋肾利肝颗粒中芍药苷含量的反相高效液相法。方法采用Krom asil C18柱,以甲醇-0.05 mol.L-1磷酸二氢钾溶液(32∶68)为流动相,紫外检测波长230 nm,流速1 m l.m in-1。结果在所使用的色谱条件下,芍药苷出峰位置未见干扰,芍药苷在24.28~121.40μg.m l-1浓度范围内线性关系良好(r=0.999 9)。滋肾利肝颗粒中芍药苷的平均加样回收率为98.32%,相对标准偏差为1.34%(n=9)。结论该法操作简便,重现性好,可作为滋肾利肝颗粒生产上质量控制的一种可信工具。
Objective To establish a method for determination of paeoniflorin in Zishen Ligan Granules by RP-HPLC. The Krom asil C18 column was used with methanol-0.05 mol.L-1 potassium dihydrogen phosphate solution (32:68) as the mobile phase, UV detection wavelength was 230 nm, and the flow rate was 1 m l.m in-1. Results Under the chromatographic conditions used, there was no interference in the peak position of paeoniflorin, and there was a good linearity in the concentration range of 24.28-121.40 μg.ml-1 (r=0.999 9). The average recovery of paeoniflorin in Zishen Ligan Granules was 98.32%, and the relative standard deviation was 1.34% (n=9). Conclusion The method is easy to operate and has good reproducibility. It can be used as a credible tool for controlling quality of Zishen Ligan Granules.