【摘 要】
：
目的:研究缺氧及复氧对hepG2细胞STC-1和STC-2的mRNA表达影响。方法:用化学缺氧剂氯化钴模拟缺氧环境,用RT-PCR法分别检测在hepG2细胞缺氧和复氧条件下STC-1、STC-2的mRNA表
【机 构】
：
广东省中医院芳村医院,广州中医药大学第二临床医学院,
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目的:研究缺氧及复氧对hepG2细胞STC-1和STC-2的mRNA表达影响。方法:用化学缺氧剂氯化钴模拟缺氧环境,用RT-PCR法分别检测在hepG2细胞缺氧和复氧条件下STC-1、STC-2的mRNA表达情况。结果:在hepG2细胞中,STC-1mRNA无论在缺氧还是复氧情况下,几乎不表达;STC-2mRNA在缺氧条件下表达升高,并随着缺氧时间延长而升高,复氧后表达又降低,并随复氧时间延长而恢复至正常。结论:STC-2与肝癌的发生发展过程密切相关。
Objective: To investigate the effects of hypoxia and reoxygenation on mRNA expression of STC-1 and STC-2 in hepG2 cells. Methods: The hypoxia environment was simulated by cobalt chloride, a chemical hypoxia reagent. The mRNA expression of STC-1 and STC-2 was detected by RT-PCR in hypoxic and reoxygenation hepG2 cells respectively. Results: In hepG2 cells, STC-1 mRNA was almost not expressed in both hypoxia and reoxygenation groups. The expression of STC-2 mRNA was increased in hypoxia and increased with hypoxia, The expression decreased again and returned to normal with prolonged reoxygenation. Conclusion: STC-2 is closely related to the occurrence and development of hepatocellular carcinoma.
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