论文部分内容阅读
目的了解泰州市沙门菌的血清型、耐药性以及基因型分布情况,为沙门菌病防治提供依据。方法将2011年泰州市腹泻病人、健康从业人员以及食品(猪肉)中分离的84株沙门菌进行生化、血清学鉴定、药敏试验以及PFGE分型,并运用BioNumeries软件分析菌株之间的相似度。结果泰州市沙门菌血清型主要以德尔卑,肠炎以及鸭沙门菌为主,其次为鼠伤寒,伦敦,阿贡纳,山夫顿堡沙门菌。药敏试验表明,腹泻病人分离株耐药性占87.5%,2耐及以上占75%;健康从业人员分离株耐药性占61.2%,2耐及以上占44.9%;食品(猪肉)分离株耐药性占71.4%,2耐及以上占32.1%。PFGE结果表明,我市7种主要沙门菌PFGE带型种类多,相似度大于50%,各亲缘关系较近,有100%同源菌株存在。结论泰州市沙门菌分离株耐药性较强,耐药谱广;PFGE是一种灵敏、可靠的研究沙门菌分子流行病学的基因分型方法,泰州市沙门菌基因型呈多态分布,但具有流行相关性。
Objective To understand the serotype, drug resistance and genotype distribution of Salmonella in Taizhou, and to provide basis for the prevention and treatment of Salmonella. Methods Eighty-four Salmonella isolated from diarrhea patients, health workers and food (pork) in Taizhou in 2011 were biochemically and serologically identified, susceptible to susceptibility testing and PFGE typing. BioNumeries software was used to analyze the similarity between the strains . Results Salmonella serotype in Taizhou City was dominated by Delps, enteritis and Salmonella typhimurium, followed by Salmonella typhimurium, Salmonella typhimurium in London, Agunga, and Shanfutun Fort. Drug susceptibility tests showed that the resistance of isolates from patients with diarrhea accounted for 87.5%, resistance to 2 and above accounted for 75%; health practitioners isolated strains accounted for 61.2%, 2 resistance and above accounted for 44.9%; food (pork) isolates Drug resistance accounted for 71.4%, 2 resistance and above accounted for 32.1%. The results of PFGE showed that there were many PFGE patterns of seven major Salmonella strains in our city with the similarity greater than 50% and close relatives, and 100% homologous strains existed. Conclusion The Salmonella isolates from Taizhou City are highly resistant and broad spectrum of drug resistance. PFGE is a sensitive and reliable method for genotyping molecular epidemiology of Salmonella. The genotypes of Salmonella in Taizhou are polymorphic, But has a popular correlation.