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目的:探讨差异甲基化杂交结合生物信息学在发现新的人上皮性卵巢癌相关基因中的应用价值。方法:采用基于芯片技术的差异甲基化杂交(differential methylation hybridization,DMH)的方法检测5例原发性上皮性卵巢癌患者癌组织(以癌旁组织为对照)的DNA异常甲基化位点。利用生物信息学,通过序列比对分析,筛选新的候选的卵巢癌相关基因。结果:上皮性卵巢癌患者癌组织与癌旁组织相比,5张差异甲基化杂交芯片共筛出异常甲基化位点62个;DNA序列比对分析发现,基因EGFLAM启动子区CpG岛(CpG island,CGI)在上皮性卵巢癌中出现低甲基化,提示该基因可能是通过启动子区的异常甲基化参与卵巢癌的发生发展。结论:将基于芯片技术的差异甲基化杂交技术与现代生物信息学相结合,可以有效地发现新的人上皮性卵巢癌相关基因。
Objective: To explore the value of differential methylation hybridization and bioinformatics in the discovery of new human epithelial ovarian cancer-related genes. Methods: DNA methylation sites of 5 cases of primary epithelial ovarian cancer were detected by differential methylation hybridization (DMH) based on microarray technique . Using bioinformatics, a new candidate ovarian cancer related gene was screened by sequence alignment analysis. RESULTS: Twenty-two sites of aberrant methylation were screened in 5 differentially methylated hybridoma cells in epithelial ovarian cancer patients compared with adjacent normal tissues. DNA sequence alignment showed that CpG island (CpG island, CGI) in epithelial ovarian cancer hypomethylation, suggesting that the gene may be involved in the development of ovarian cancer through abnormal promoter methylation. Conclusion: The combination of differential methylation hybridization technology based on chip technology and modern bioinformatics can effectively discover new human epithelial ovarian cancer related genes.