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目的测试两种装载方法实验动物垫料压力蒸汽灭菌的条件,并比较灭菌效果。方法分别用笼盒重叠法和袋装法装载刨花垫料,121℃、20 min压力蒸汽灭菌,用化学指示卡测试灭菌条件。分别取两种装载方法灭菌前后的垫料10 g放入灭菌生理盐水中充分摇匀,取1 ml接种于血平板,37℃、48 h培养,以血平板上是否生长菌落为灭菌合格与否的判定标准。结果与标准色比较,笼盒重叠法未能全部达到灭菌条件,其灭菌合格率为83.3%;袋装法各部位均达到灭菌条件,其灭菌合格率为100.0%。结论袋装法灭菌效果优于笼盒重叠法。
Objective To test the conditions of pressure steam sterilization of litter in two kinds of loading methods and compare the sterilization effect. Methods Cage packing method and bagging method were respectively used to load the wood shavings, which were sterilized by steam at 121 ℃ for 20 min. The sterilization conditions were tested by chemical indicator. Respectively, take two loading method before and after sterilization of litter 10 g into sterile normal saline and shake well, take 1 ml of inoculated on the blood plate, 37 ℃, 48 h culture to grow on the blood plate colonies for sterilization Qualified or not to determine the standard. Results Compared with the standard color, the cage overlap method failed to achieve all the sterilization conditions, the sterilization rate was 83.3%; bagging method to achieve sterilization conditions in all parts of the sterilization rate of 100.0%. Conclusion The bagging method is better than cage overlap method.