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目的:研究液压冲击性脑损伤后成年大鼠脑组织碱性成纤维细胞生长因子(bFGF)表达时程和空间分布的变化。方法:制作液压冲击性脑损伤模型,免疫组织化学法动态检测内源性bFGF的变化。结果:在正常脑组织中,bFGF免疫活性低水平表达,且主要位于胶质细胞核及神经元细胞质中。液压冲击伤后bFGF免疫活性增强,聚集核周边。此外,部分bFGF免疫活性聚集于神经元周围间隙中半定量分析显示液压冲击伤后3d,大脑皮质bFGF阳性细胞数量较正常对照组升高4.8倍;伤后7 d,bFGF免疫活性达高峰,伤后30 d消失。结论:液压冲击伤后,损伤的胶质细胞和神经元暂短地合成bFGF,并以旁分泌和自分泌形式起作用。
Objective: To study the time course and spatial distribution of basic fibroblast growth factor (bFGF) expression in brain tissue of rats after fluid percussion brain injury. Methods: The model of hydraulic shock brain injury was made and the changes of endogenous bFGF were detected by immunohistochemical method. Results: In normal brain tissue, the bFGF immunoreactivity was expressed at a low level and mainly located in the glial cell nucleus and neuronal cytoplasm. After bashing, the bFGF increased its immunocompetence and gathered around the nucleus. In addition, semi-quantitative analysis of bFGF immunoreactivity gathered in the space around neurons showed that the number of bFGF positive cells in cerebral cortex increased 4.8 times than that of normal control group on the 3rd day after hydraulic shock injury. At 7 d after injury, the bFGF immunocompetent reached its peak, After 30 days disappeared. CONCLUSIONS: Injured glial cells and neurons transiently synthesize bFGF following a hydraulic shock injury and function in a paracrine and autocrine form.