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以黄瓜20个栽培品种的子叶、子叶节和下胚轴为试材,进行发芽、分化和诱导分化不定芽试验,建立最适于黄瓜品种的再生体系。结果表明:供试的20个黄瓜品种中,“农家乐”、“神农春四F1”2个品种的发芽情况显著优于其它品种,发芽率分别为66.7%和83.3%;在MS+0.5mg/mL 6-BA培养基中,20个黄瓜品种不同类型外植体的分化效率以子叶节最佳;“农家乐”、“神农春四F1”使用MS+0.5mg/L 6-BA+1.0mg/L AgNO3培养基,芽的增殖系数最高,诱导率分别为50.0%、56.3%。该试验根据不同品种的发芽率以及诱导愈伤组织和芽的分化,建立了黄瓜快速、高频再生体系,为进一步研究黄瓜的遗传转化和基因工程育种提供参考依据。
Cotyledon, cotyledonary node and hypocotyls of 20 cultivated cucumber cultivars were used as materials for germination, differentiation and induction of adventitious buds. The optimal regeneration system for cucumber cultivars was established. The results showed that the germination rate of two varieties of “Nongjiale” and “Shennongchunsi F1” was significantly better than that of other cultivars, with the germination rates of 66.7% and 83.3%, respectively. Among the 20 cucumber varieties tested, + 0.5mg / mL 6-BA medium, the differentiation efficiency of different types of explants of 20 cucumber cultivars was the best in cotyledonary node; “” “Nongjiale” and “Shennongchun 4 F1” L 6-BA + 1.0mg / L AgNO3 medium. The buds had the highest proliferation rate with the induction rates of 50.0% and 56.3%, respectively. According to the germination rate of different varieties and the differentiation of callus and bud, the rapid and high-frequency regeneration system of cucumber was established in this experiment to provide reference for further research on genetic transformation and gene engineering breeding of cucumber.