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１９９１～１９９３年在Ｍａｒｔｏｎｖａｓａｒ的苗圃进行人工侵染试验，研究腥黑穗病对本地繁育的小麦品种和含有已知抗性基因的单基因品系（分别含Ｂｔ１～Ｂｔ１０）的侵染，并于１９９３年用健康小麦幼苗和感染了腥黑穗病的幼苗在Ｍａｒｔｏｎｖａｓａｒ的人工气候室进行冻害试验。在试验期间，当地的病原种群对Ｂｔ９和Ｂｔ１０基因无致病力，而对Ｂｔ７则致病力很强。含有抗病基因Ｂｔ４、Ｂｔ５、Ｂｔ６和Ｂｔ８的单基因品系具有良好的抗病性，含Ｂｔ１、Ｂｔ２和Ｂｔ３的单基因品系只有中度的抗性。在本地的小麦品种中，只有三个被证实具有一定的抗性（即“Ｍａｒｔｏｎｖａｓａｒｉ１７”、“Ｍａｒｔｏｎｖａｓａｒｉ２３”和“Ｆａｔｉｍａ２”），其余的表现感病或严重感病。侵染腥黑穗病的植株比健康植株抗寒性严重降低，超过了品种的平均数。品种被腥黑穗病侵染的程度与冻害致死率成显著正相关（ｒ＝０．８９０），而含有不同抗性基因（Ｂｔ）的单基因品系在这方面则不相关。
Artificial infection experiments were conducted in nurseries of Martonvasar from 1991 to 1993 to study the effects of tsetse on locally-bred wheat varieties and single-line strains containing known resistance genes (Bt1-Bt10, respectively) and in 1993 In winter, young healthy wheat seedlings and seedlings infected with tauluats were subjected to freeze injury tests in Martonvasar’s climate chamber. During the experiment, local pathogenic populations showed no virulence to the Bt9 and Bt10 genes, but virulence to Bt7. The single gene lines containing the resistance genes Bt4, Bt5, Bt6 and Bt8 have good disease resistance, and the single gene lines with Bt1, Bt2 and Bt3 have only moderate resistance. Of the local wheat cultivars, only three proved to be resistant (ie, “Martonvasari 17”, “Martonvasari 23” and “Fatima 2”) and the remaining were susceptible or severely affected. Cold-affected plants infected with tardiness than the healthy plants severely reduced cold resistance, exceeding the average number of varieties. There was a significant positive correlation (r = 0.890) between the susceptible varieties and the lethality of frostbite. However, the single gene lines with different resistance genes (Bt) were not related in this respect.