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利用Rep-PCR的两对引物Pot2-1/Pot2-2和PMC1-2/PMC1-3、177条RAPD引物和MGR586为探针的3种RFLP分子技术对温室的9个和自然病圃上的5个致病性突变菌株进行分析。3种技术鉴定了温室9个菌株与其起始菌株的DNA指纹基本一致,检测到7个菌株DNA条带缺失1~3条,2个菌株没有变化;分析了病圃上的侵染携带抗病基因Pi-1和Pi-2的5个突变株的起源,依据DNA指纹和致病类型证明后者起源于具有无毒基因Avr-1的菌株,排除了外来菌株的漂移作用,证实了突变是稻瘟病菌(Magnaporthegrisea)进化的主要动力。
Three pairs of primers of Rep-PCR Pot2-1 / Pot2-2 and PMC1-2 / PMC1-3, 177 RAPD primers and MGR586 probe were used to probe the effect of three RFLP molecular techniques on the greenhouse and in natural nurseries Five pathogenic mutant strains were analyzed. The DNA fingerprinting of the 9 strains in the greenhouse was basically the same as that of the original strain. The results showed that there were 1 to 3 DNA bands deletion in 7 strains and no change in 2 strains. The origin of five mutants of Pi-1 and Pi-2 genes proved that the latter originated from Avr-1-free strain according to DNA fingerprinting and pathogenicity, which excluded the extrinsic migration of foreign strains and confirmed that the mutation was Magnaporthe grisea evolution of the main driving force.