论文部分内容阅读
目的 :探讨慢性牙周炎患者治疗前、后血清中降钙素基因相关肽(calcitonin gene-related peptide,CGRP)水平的变化及意义。方法:随机选择2014年8月—2015年6月在我院牙周科就诊的健康对照组30例,轻度、中度、重度牙周炎患者各30例,抽取空腹外周静脉血,离心、分离上清液,运用酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA)检测CGRP浓度。慢性牙周炎患者轻度、中度、重度3组分别于基础治疗后3个月再次检测CGRP浓度。采用SPSS 22.0软件包对数据进行统计学分析。结果:ELISA检测结果显示,健康组静脉血中CGRP的含量显著高于牙周炎患者。随着牙周炎炎症程度加重,静脉血中CGRP的含量依次降低,重度牙周炎患者的含量最低,差异显著(P<0.01);轻度、中度、重度牙周炎患者基础治疗3个月后,血清中CGRP含量相比同组治疗前显著升高(P<0.05),治疗后牙周炎患者组间CGRP含量相比差异无显著性(P>0.05)。结论:静脉血中CGRP的含量与慢性牙周炎存在相关性,其含量随慢性牙周炎逐渐加重而降低,推测CGRP可能参与慢性牙周炎的发生、发展过程。慢性牙周炎治疗后血清中CGRP含量明显增加,CGRP可作为慢性牙周炎患者治疗效果的临床检测指标。
Objective: To investigate the changes and significance of serum levels of calcitonin gene-related peptide (CGRP) in patients with chronic periodontitis before and after treatment. Methods: Thirty patients were randomly selected from the periodontal department of our hospital from August 2014 to June 2015. Thirty patients with mild, moderate and severe periodontitis were randomly divided into three groups: fasting peripheral venous blood, centrifugation, The supernatant was separated and the concentration of CGRP was detected by enzyme-linked immunosorbent assay (ELISA). Patients with chronic periodontitis were tested again for CGRP concentration in the mild, moderate and severe groups at 3 months after the initial treatment. Data were statistically analyzed using SPSS 22.0 software package. Results: The results of ELISA showed that the content of CGRP in venous blood of healthy group was significantly higher than that of periodontitis patients. With the aggravation of periodontitis, the content of CGRP in venous blood decreased in turn, and the content of severe periodontitis was the lowest, with significant difference (P <0.01). The basic treatment of mild, moderate and severe periodontitis was 3 The level of CGRP in serum was significantly higher than that of the same group before treatment (P <0.05). There was no significant difference in the content of CGRP between the two groups after treatment (P> 0.05). Conclusion: The content of CGRP in venous blood is correlated with chronic periodontitis. The content of CGRP in chronic venous blood decreases with the progression of chronic periodontitis. It is speculated that CGRP may be involved in the occurrence and development of chronic periodontitis. After treatment of chronic periodontitis serum CGRP levels increased significantly, CGRP can be used as clinical indicators of treatment of patients with chronic periodontitis.