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目的对不同温度下人体肝细胞DNA降解状态进行多参数定量分析,得出温度相关的多元回归方程。方法选取13例已知PMI的人体离体肝脏,在死后13～34h内,分别在10℃、20℃及30℃条件下,每隔1h取材,进行细胞学涂片,Feulgen-Vans染色,选用图像分析系统检测几何参数(ID)及灰度参数(AOD)指标,对不同温度的检测指标进行多元回归分析,得出相应的回归方程。结果温度相关的肝细胞DNA降解推断死亡时间的回归方程:Y=25.789-0.656XT-41.958XAOD+3.262XID(XT=10,20,30)。结论温度及反映DNA含量的各个参数与PMI存在较强的相关性,可以将温度作为参数引入方程用于推断PMI。
Objective To quantitatively analyze the DNA degradation status of human hepatocytes at different temperatures and obtain the temperature-dependent multiple regression equation. Methods Thirteen PMI isolated human liver tissues were isolated from the liver of PMI patients at 13 to 34 hours after death. The cells were stained with cytology and Feulgen-Vans at 1 ℃, 10 ℃, 20 ℃ and 30 ℃ respectively. The image analysis system was used to detect geometric parameters (ID) and gray level parameters (AOD) indicators, multiple regression analysis of the detection indicators of different temperatures, the corresponding regression equation was obtained. Results Regression equation of temperature-dependent DNA degradation deduced death time: Y = 25.789-0.656XT-41.958XAOD + 3.262XID (XT = 10,20,30). Conclusion The temperature and DNA parameters reflect the strong correlation with PMI. The temperature can be introduced into the equation as a parameter to infer the PMI.