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蓖麻毒素（ｒｉｃｉｎ） 的抗癌作用是，借助于其抑制细胞的蛋白质合成而导致细胞死亡的毒性作用，但其缺点是其分子中的Ｂ 链含有半乳糖结合位点， 可非特异性的与真核细胞结合。本研究通过采用双功能剂ＳＰＤＰ 修饰ｒｉｃｉｎ 后，可达到部分或完全破坏ｒｉｃｉｎ 分子结构中的半乳糖结合位点。另外，用ＭＴＴ 法比较了修饰后的ｒｉｃｉｎ 与未修饰ｒｉｃｉｎ的细胞毒性，同时检测并比较了两者对细胞因子ＴＮＦα和ＩＬ１β的诱生作用。结果表明，修饰后的ｒｉｃｉｎ 与未修饰的ｒｉｃｉｎ 相比较，两者对Ｕ９３７ 细胞的毒性在时间效应和剂量效应上没有显著性差异。修饰后的ｒｉｃｉｎ 诱生ＴＮＦα的量比未修饰的ｒｉｃｉｎ 多，且时间早；而对于诱生ＩＬ１β来说，在２４ｈ 前两者差别不大，在４８ｈ 时，修饰的ｒｉｃｉｎ 则明显高于未修饰的ｒｉｃｉｎ 。提示ｒｉｃｉｎ 经ＳＰＤＰ 修饰后，毒性改变不大，但其诱生细胞因子ＴＮＦα和ＩＬ１β的量则高于未修饰的ｒｉｃｉｎ 。
The anti-cancer effect of ricin is the toxic effect of cell death caused by its inhibition of protein synthesis in cells, but its disadvantage is that the B chain in its molecule contains a galactose binding site, which can be nonspecific. Eukaryotic cell binding. In this study, the galactose binding site in the structure of ricin was partially or completely destroyed by modifying ricin with bifunctional SPDP. In addition, the cytotoxicity of modified ricin and unmodified ricin was compared using MTT assay, and the induction of cytokines TNF-α and IL-1β was detected and compared. The results showed that compared with the unmodified ricin, there was no significant difference in the time and dose effects of the modified ricin on U937 cells. The modified ricin induces more TNFα than unmodified ricin, and the time is early; while for IL1β, the difference is not significant before 24h, and the modified ricin is obvious at 48h. Higher than unmodified ricin. It is suggested that the toxicity of ricin modified by SPDP is not significant, but the amount of induced cytokines TNF-α and IL-1β is higher than that of unmodified ricin.