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利用体外灌流内皮细胞(EC)单层的模型和细胞形态参数自动分析的软件,研究异丙肾上腺素(IPN)对血小板激活因子(PAF)所致EC单层通透性和细胞形态变化的影响.白蛋白对EC单层的屏障功能有增强作用.PAF(10~(-8)mol/L)明显增高EC单层的通透性,而且使细胞面积缩小,细胞间距增大.预先用IPN(10~(-4)mol/L)处理可完全阻断PAF引起的EC层通透性和形态学变化,说明EC收缩和细胞间隙开放是PAF增高血管壁通透性的重要机制.IPN通过稳定细胞形态,阻止细胞间隙开放,抑制PAF引起的EC单层通透性增高.
The effects of isoproterenol (IPN) on the monolayer permeability and morphological changes of EC induced by platelet activating factor (PAF) were studied by using the model of monolayer of perfused endothelial cells (EC) and the automatic analysis of cell morphology parameters Albumin could enhance the barrier function of EC monolayer.PAF (10 ~ (-8) mol / L) significantly increased the permeability of EC monolayer and reduced the area of cells and increased the intercellular distance.Using IPN (10 -4 mol / L) could completely block the permeability and morphological changes of EC layer induced by PAF, indicating that EC contraction and cell gap opening are important mechanisms of PAF increasing vascular permeability. Stabilize cell morphology, prevent cell gap opening, inhibit PAF-induced EC monolayer permeability increased.