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目的 探讨胶质瘤促血管生成素2 (Ang2) 基因在脑胶质瘤的表达及其临床意义。方法 采用逆转录聚合酶链反应(RT PCR) 和链酶亲和素过氧化物酶复合物SABC法检测52例人脑胶质瘤和8例正常脑组织中Ang2基因表达。结果 50 例脑胶质瘤和8 例正常脑组织均可表达Ang2 mRNA片段。高恶度胶质瘤Ang2 mRNA及蛋白表达显著高于低恶度胶质瘤(P<0 01); 低恶度胶质瘤Ang2 mRNA及蛋白表达显著高于正常脑组织(P<0 05)。免疫组化染色显示, Ang2蛋白主要分布在高恶度胶质瘤组织的瘤细胞和内皮细胞, 低恶度胶质瘤呈低水平表达, 正常脑组织不表达。结论 Ang2的表达与胶质瘤的分级密切相关, 可能对胶质瘤的恶性演进起促进作用。
Objective To investigate the expression of angiopoietin 2 (Ang2) gene in glioma and its clinical significance. Methods The expression of Ang2 gene was detected in 52 human gliomas and 8 normal brain tissues by reverse transcription polymerase chain reaction (RT PCR) and streptavidin-peroxidase complex SABC method. Results Ang2 mRNA was expressed in 50 gliomas and 8 normal brain tissues. The expression of Ang2 mRNA and protein in high grade gliomas was significantly higher than that in low grade gliomas (P <0.01). The expression of Ang2 mRNA and protein in low grade gliomas was significantly higher than that in normal brain tissues (P <0.05) . Immunohistochemical staining showed that Ang2 protein mainly distributed in the tumor cells and endothelial cells of high grade gliomas, low grade glioma was low level expression, normal brain tissue was not expressed. Conclusion The expression of Ang2 is closely related to the grade of glioma, which may promote the malignant progression of glioma.