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目的 通过观察在再灌流时实施头部重点低温对兔脑神经元形态学变化的影响 ,判断它能否减轻神经元的缺血再灌流损伤 ,进而探讨相应的机理。方法 将 16 8只新西兰兔随机分为 3组 ,Ⅰ组为未缺血组 [n =2 4 ,(38± 0 5 )℃ ];Ⅱ组为常温再灌流组 [n =72 ,(38± 0 5 )℃ ];Ⅲ组为头部重点低温治疗组 [n =72 4 ,(2 8± 0 5 )℃ ,开始再灌流时实施头部重点低温〗 ,后两组动物的 3个亚组 (n =2 4 )在脑完全性缺血 30分钟后分别再灌流 30、180和 36 0分钟。同时检测了 12个脑区的四类神经元 (正常、轻伤、重伤和坏死 )和脑组织中 14项生化参数的变化。结果 与Ⅰ组相比 ,Ⅱ组正常神经元数量进行性下降、重伤和坏死神经元进行性增多 (P <0 0 1) ;这分别与生化参数VIP、β EP、PGI2 、T3 、T4 与Na+ ,K+ ATPase ,Glu、AVP与TXB2 /PGI2 ,和T4 、Na+ ,K+ ATPase、GLN、T3 与VIP相关 (P <0 0 5 ) ;Ⅲ组数量较Ⅱ组正常神经元数量大幅度增多、重伤和坏死神经元明显下降 (P <0 0 1) ,与这三类神经元数量变化相关的参数是Ca2 + ,Mg2 + ATPase (P <0 0 1)。结论 再灌流时实施的 6小时头部重点低温确能减轻兔脑神经元损害 ,作用途径为锐减重伤和坏死神经元的产生 ,促进轻伤神经元的修复 ,保护受累神经元
OBJECTIVE: To observe whether neuronal damage of neurons can be alleviated by observing the effect of focal head hypothermia on neuronal morphological changes in rabbit brain during reperfusion, and then to explore the corresponding mechanism. Methods 168 New Zealand white rabbits were randomly divided into 3 groups: group Ⅰ was not ischemic (n = 24, 38 ± 0 5 ℃); group Ⅱ was normal temperature reperfusion group (n = 72, 38 ± (N = 72 4, (2 8 ± 0 5) ℃), group Ⅲ was the key low temperature treatment group (n = 72 4, (2 8 ± 0 5) ℃, (n = 2 4) were reperfused 30, 180 and 36 0 minutes after 30 minutes of complete cerebral ischemia, respectively. At the same time, the changes of four kinds of neurons (normal, minor injuries, serious injuries and necrosis) in 12 brain regions and 14 biochemical parameters in brain were detected. Results Compared with group Ⅰ, the number of normal neurons in group Ⅱ decreased progressively, and the number of severely injured and necrotic neurons increased progressively (P <0.01). These results were compared with those of biochemical parameters VIP, β EP, PGI2, T3, T4 and Na + (P <0 05), K + ATPase, Glu, AVP, TXB2 / PGI2, T4, Na +, K + ATPase, GLN and T3 significantly increased compared with those in group Ⅱ The number of necrotic neurons decreased significantly (P <0.01). The parameters related to the number of these three neurons were Ca2 + and Mg2 + ATPase (P <0.01). CONCLUSION: The 6-hour head-key hypothermia during reperfusion can indeed reduce the damage of neurons in rabbit brain by reducing sharply injured and necrotic neurons, promoting the repair of injured neurons and protecting the involved neurons