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本文报告建立高效液相色谱法测定我国发展成功的呋苄青霉素体液中药物浓度。本法用氯呋苄青霉素作为内标物。固定相为Partisil ODS,10μm;流动相为甲醇/磷酸盐缓冲液,紫外检测波长为275nm,流动相流速为1.5ml/min。方法学多种指标考核表明该法灵敏、特异、准确、精密,可用于呋苄青霉素临床药理研究。正常人静脉注射呋苄青霉素1.0克,药代动力学符合二室开放式模型。药代动力力学方程为Ct=145.8~(e-2?47t)+18.5e~(-0?64t)。即刻血清峰浓度为178.3mg/L,消除半衰期T_(1/2β)为1.08小时,表观分布容积Vd为17.69升;24小时尿中回收原型药物为给药剂量的29.63%。
This paper reports the establishment of high performance liquid chromatography to determine the development of China’s success rate of drug concentration of furosemide body fluids. Clofibrate penicillin as an internal standard. The stationary phase was Partisil ODS, 10 μm. The mobile phase consisted of methanol / phosphate buffer with a UV detection wavelength of 275 nm and a mobile phase flow rate of 1.5 ml / min. Methodology A variety of indicators examination showed that the method is sensitive, specific, accurate and precise, and can be used for furosemide clinical pharmacology. Normal intravenous furosemide 1.0 g, pharmacokinetics in line with two-compartment open model. The pharmacokinetic equation of the drug is Ct = 145.8 ~ (e-2 ~ 47t) + 18.5e ~ (-0 ~ 64t). Immediate peak serum concentration of 178.3mg / L, elimination half-life T_ (1 / 2β) of 1.08 hours, the apparent distribution volume Vd was 17.69 liters; 24 hours urinary recovery of the prototype drug dose 29.63%.