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本文利用细胞培养技术和琥珀酸脱氢酶组织化学技术检测了29种合金和6种金属的细胞毒性。材料和方法按照主要原子的含量,15种金、7种钯、4种银、1种钛、1种铬和1种铁合金及金、钯、钛、银、镍和铜等金属用于实验。标本制成直径5mm、厚3mm的圆盘状,在其一面制备直径2.5mm、长10mm的柄。标本清洁后置于24孔无菌培养板上,真空保存1个月后加入细胞。细胞培养实验按照Craig和Hanks描述的方法进行。聚四氟乙烯用作阴性对照,可塑聚氯乙烯用作阳性对照,若干空白孔用作细胞最大生长力及琥珀酸脱氢酶活性的对照孔。采用抑菌环的直径、光密度和染色细胞的密度对所得结果进行评价. 结果和讨论金、钯和钛的细胞毒性最小,无抑菌环形成,具有很好的生物相容性。银和镍依次次之,铜
In this paper, cytotoxicity of 29 kinds of alloys and 6 kinds of metals was detected by cell culture technique and succinate dehydrogenase histochemical technique. Materials and Methods According to the content of main atoms, 15 kinds of gold, 7 kinds of palladium, 4 kinds of silver, 1 kind of titanium, 1 kind of chromium and 1 kinds of ferroalloy and the metals of gold, palladium, titanium, silver, nickel and copper were used in the experiment. The specimen was formed into a disc shape having a diameter of 5 mm and a thickness of 3 mm, and a handle having a diameter of 2.5 mm and a length of 10 mm was prepared on one side thereof. The specimens were placed on a 24-well sterile culture plate after cleaning and added to the cells after 1 month in vacuum. Cell culture experiments were performed as described by Craig and Hanks. Polytetrafluoroethylene was used as a negative control, plasticized polyvinyl chloride was used as a positive control, and a number of blank wells were used as control wells for maximum cell viability and succinate dehydrogenase activity. The results were evaluated by the diameter of the bacteriostatic ring, the optical density and the density of the stained cells.Results and Discussion Gold, palladium and titanium have the lowest cytotoxicity, no antibacterial ring formation and good biocompatibility. Followed by silver and nickel, copper