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Objective: The aim of our study was to explore the correlations between both the genes, evolution and prognosis of the disease by detecting the expression of MN1 (meningioma 1) gene and PTEN (phosphatase and tensin homolog) gene in patients with acute myeloid leukemia (AML). Method: MN1 and PTEN mRNA were detected by reverse transcription-PCR in mononuclear cells of 38 patients with AML and 13 patients with normal bone marrow. Results: Positive rates of MN1 and PTEN genes were 76.3% and 60.5% respectively in bone marrow mononuclear cells. The expression level of MN1 mRNA for the de novo group increased comparing with that for normal control group (P < 0.05), the expression level of PTEN mRNA for de novo group decreased obviously comparing with that for normal control group (P < 0.01), MN1 mRNA level decreased in remission group, while PTEN mRNA level increased comparing with that in de novo group (P < 0.05). MN1 mRNA level increased and PTEN mRNA decreased in relapsed group comparing with that in control group, and their difference was statistical significance (P < 0.05). The two genes' levels had negative correlations in acute myeloid leukemia (r = –0.314, P < 0.05). Conclusion: There is close correlations between expression of MN1 and PTEN genes and the prognosis and occurrence of acute myeloid leukemia, and their expression can be taken as significant indexes to access the de novo, relapse and prognosis of acute myeloid leukemia.