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目的研究纳米磁性四氧化三铁(Nano-Fe3O4)和纳米二氧化钛(Nano-TiO2)在小鼠体内的短期与远期分布,为其合理开发利用提供参考依据。方法将2种纳米材料分别腹腔注射到小鼠体内,用原子吸收光谱仪(AAS)和电感耦合等离子体原子发射光谱仪(ICP-OES)检测其在小鼠体内重要器官中的短期和远期分布,进行定量分析,并进行病理组织学检查。结果Nano-Fe3O4在小鼠体内的分布依次为心、脾、肝、肺、肾、脑。能通过血脑屏障,但尚未引起组织的病理学改变。长期注射无蓄积。本实验中Nano-Fe3O4的最大耐受量>100mg/kg。Nano-TiO2在小鼠脾脏和肝脏分布较多,能通过血睾屏障,但尚未引起组织的病理学改变。长期注射微有蓄积。本实验中Nano-TiO2的最大耐受量>300mg/kg,其小鼠体内脏器摄取量均不如Nano-Fe3O4。结论Nano-Fe3O4和Nano-TiO2毒副反应风险低,有较好的应用前景。
Objective To study the short-term and long-term distribution of Nano-Fe 3 O 4 and Nano-TiO 2 in mice and to provide a reference for their rational development and utilization. Methods Two kinds of nanomaterials were injected intraperitoneally into mice respectively. The short-term and long-term distribution of these two kinds of nanomaterials in mice were detected by atomic absorption spectroscopy (AAS) and inductively coupled plasma atomic emission spectrometry (ICP-OES) Quantitative analysis, and histopathological examination. Results Nano-Fe3O4 distribution in mice followed by heart, spleen, liver, lung, kidney and brain. Can pass the blood-brain barrier, but has not caused the pathological changes in the tissue. Long-term injection without accumulation. In this experiment, the maximum tolerance of Nano-Fe3O4> 100mg / kg. Nano-TiO2 in the spleen and liver of mice more distribution, can pass the blood testis barrier, but has not yet caused the pathological changes of the tissue. Long-term injection of micro accumulation. In this experiment, the maximum tolerated dose of Nano-TiO2 is> 300mg / kg. The uptake of organ in mice is not as good as Nano-Fe3O4. Conclusion Nano-Fe3O4 and Nano-TiO2 have low toxicity and good application prospects.