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目的探索检测卵巢癌石蜡块组织中Her-2/neu状态的新方法。方法选取69例卵巢癌组织蜡块,用实时PCR检验卵巢癌组织中的Her-2/neu基因的扩增情况,用免疫组化检测其蛋白表达情况,将两者结果进行比较,并用FISH试验来验证其结果。结果肿瘤组织中Her-2/neu的扩增如果以相对基因拷贝数(肿瘤细胞内基因拷贝数与正常二倍体卵巢组织细胞基因拷贝数的比率)≥1.5或≥2为截取值,本组卵巢癌组织中Her-2/neu的扩增率分别为26.1%(18/69)、15.9%(11/69);本组卵巢癌组织中Her-2/neu免疫组化为++~+++者为28.99%(20/69);两种检测方法的结果具有很高的一致性。结论实时PCR简便、客观、高效,可望成为卵巢癌石蜡组织中除免疫组化外检测Her-2/neu变化的一种备选方法。
Objective To explore a new method for detecting the state of Her-2 / neu in paraffin blocks of ovarian cancer. Methods The expression of Her-2 / neu gene in ovarian cancer tissues was detected by real-time PCR. The expression of Her-2 / neu gene in 69 cases of ovarian cancer tissues was detected by immunohistochemistry. The results were compared with FISH test To verify the result. Results Expansion of Her-2 / neu in Tumor Tissues If the relative gene copy number (the ratio of the gene copy number in the tumor cells to the gene copy number in the normal diploid ovarian tissue cells) is ≥1.5 or ≥2, The positive rates of Her-2 / neu in ovarian cancer tissue were 26.1% (18/69) and 15.9% (11/69) ++ was 28.99% (20/69); the results of the two methods were highly consistent. Conclusion Real-time PCR is simple, objective and efficient and it is expected to be an alternative method to detect the changes of Her-2 / neu in ovarian cancer paraffin tissues besides immunohistochemistry.