论文部分内容阅读
目的探索研究不同分型的子宫内膜异位症患者体内腹腔液中端粒酶及端粒酶逆转录酶的表达及对子宫内膜异位症发展的影响。方法在腹腔镜下收集64例子宫内膜异位症手术患者体内腹腔液,应用酶联免疫吸附实验(ELISA)及荧光原位杂交实验(FISH)方法分别检验收集的腹腔液中端粒酶的阳性表达及端粒酶逆转录酶(hTERC)的扩增情况,并与对照组(盆腔炎患者)相比较。结果应用ELISA方法检验出子宫内膜异位症组腹腔液中端粒酶阳性率与对照组差异无统计学意义;FISH实验检验出子宫内膜异位症组腹腔液中人类端粒酶逆转录酶扩增阳性率0-Ⅱ期与对照组差异无统计学意义;Ⅲ-Ⅳ期患者于对照组差异有统计学意义。结论子宫内膜异位症患者腹腔液中端粒酶逆转录酶表达均高于实验对照组,其可能与异位子宫内膜的恶变及增值活性较高相关。
Objective To investigate the expression of telomerase and telomerase reverse transcriptase in peritoneal fluid of patients with endometriosis of different types and their effects on the development of endometriosis. Methods The peritoneal fluid of 64 patients with endometriosis was collected by laparoscopy. The activity of telomerase in the collected peritoneal fluid was detected by ELISA and FISH respectively. Positive expression and the amplification of telomerase reverse transcriptase (hTERC), and compared with the control group (pelvic inflammatory disease patients). Results The positive rate of telomerase in peritoneal fluid of endometriosis group was not significantly different from that of the control group by ELISA. The telomerase reverse transcription of peritoneal fluid in endometriosis group was detected by FISH The positive rate of enzyme amplification in stage 0-Ⅱ was not significantly different from that in control group. The difference in stage Ⅲ-Ⅳ was statistically significant in control group. Conclusions The expression of telomerase reverse transcriptase in peritoneal fluid of patients with endometriosis is higher than that of the experimental control group, which may be related to the malignant transformation of ectopic endometrium and the higher value-added activity.