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近年来,危害小黑杨Populussimonii×P.nigra的害虫发生严重,给林业生产造成很大损失。为了提高小黑杨的抗虫能力,避免使用杀虫剂带来的污染,用农杆菌介导法将澳大利亚漏斗蛛Atraxrobustus的毒蛋白基因和苏云金芽孢杆菌CryⅠA(b)基因C末端的融合基因BGT转化入小黑杨。PCR和Southern印记分析转基因植株,结果表明,BGT杀虫基因已经整合在小黑杨基因组上。活性实验表明,取食转基因杨树6天和9天后,舞毒蛾Lymantriadispar2龄幼虫的校正死亡率分别是37.0%和92.6%。方差分析表明取食转基因和对照杨树的舞毒蛾幼虫体重差异显著。这些结果显示转基因杨树上的舞毒蛾的发育速率受到影响。
In recent years, the pest that endangered populus Populussimonii × P.nigra has been seriously damaged, causing great losses to forestry production. In order to improve the resistance of Populus tomentosa and avoid the pollution caused by insecticides, Agrobacterium tumefaciens-mediated method was used to combine the toxic protein gene of Australian tracheid Atraxrobustus and the C-terminal fusion gene of Bacillus thuringiensis CrylA (b) gene BGT Transferred into the black poplar. PCR and Southern blot analysis of transgenic plants, the results show that, BGT insecticidal genes have been integrated in the black poplar genome. The activity tests showed that the corrected mortality rates of Lymantriadis par second-instar larvae of Gypsy moth were 37.0% and 92.6% after feeding on transgenic poplars for 6 days and 9 days, respectively. Analysis of variance showed significant differences in body weight between Gypsy moth larvae fed on transgenic and control poplars. These results show that the development of Gypsy moth on transgenic poplars is affected.