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AIM:To investigate the relationship among gastrin,somatostatin,G and D cells in gastric ulcer and in itshealing process in rats.METHODS:Fourty-nine Wistar rats were divided into 7groups.The gastric ulcer model was induced by acetic acidsuccessfully.The gastrin and the somatostatin in ratplasma,gastric fluid and antral tissue were measured byradioimmunoassay(RIA).G and D cells in antral mucosawere analyzed with polyclonal antibody of gastrin andsomatostatin by immunohistochemical method andQuantimet 500 image analysis system.RESULTS:In gastric ulcer,the level of gastrin in plasma,gastric fluid,and antral tissue increased,that ofsomatostatin declined,and the disorder gradually recoveredto the normal level in the healing process.Immunohistochemical technique of G and D cells in antralmucosa demonstrated that the number of G cells increasedand that of D cells decreased,both areas of G and D cellsdeclined,the ratio of number and area of G/D increased ingastric ulcer,and the disorder gradually recovered in thehealing process.CONCLUSION: In gastric ulcer, the increased gastrin secreted by G cells, the declined somatostatin secreted by D cells, and the disordered G/D cell ratio can lead to gastrointestinal dysfunction.
AIM: To investigate the relationship among gastrin, somatostatin, G and D cells in gastric ulcer and in its healing process in rats. METHODS: Fourty-nine Wistar rats were divided into 7 groups. The gastric ulcer model was induced by acetic acid successfully. The gastrin and the somatostatin in rat plasma, the gastric fluid and antral tissue were measured by radioimmunoassay (RIA). G and D cells in antral mucosawere analyzed with polyclonal antibody of gastrin and somatostatin by immunohistochemical method and Quantimer 500 image analysis system .RESULTS: In gastric ulcer, the level of gastrin in plasma, gastric fluid, and antral tissue increased, that ofsomatostatin declined, and the disorder gradually recovered to the normal level in the healing process. Immunohistochemical technique of G and D cells in antral mucosa demonstrated that the number of G cells increased and that of D cells decreased , both areas of G and D cells defined, the ratio of number and area of G / D increased ingastric ulcer, and the disorder gradual ly recovered in thehealing process. CONCLUSION: In gastric ulcer, the increased gastrin secreted by G cells, the declined somatostatin secreted by D cells, and the disordered G / D cell ratio can lead to gastrointestinal dysfunction.