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应用SRAP技术对“锦绣”黄桃、“丰黄”黄桃及其相应变异株系等7个材料进行了分析。从40对引物组合中筛选出2对引物在“丰黄”及变异株“锦黄”之间有明显扩增带差异,表明遗传变异已发生。分别纯化、克隆、测序这些特异条带,分析序列预测所示基因,并设计引物将其转化为稳定SCAR标记。应用特异的SCAR标记可快速检测鉴定相应变异株,进一步验证了变异的存在。试验未筛选到合适引物可区分“锦绣”及其变异株系。
SRAP technique was used to analyze seven materials including “Peach Blossom”, “Peach Blossom”, “Huang Feng” and its corresponding mutant lines. Two pairs of primers were screened out from 40 pairs of primers, which showed significant differences between “Fenghuang” and mutant “Jinhuang”, indicating that genetic variation had occurred. The specific bands were purified, cloned and sequenced respectively. The genes predicted by sequence analysis were analyzed and primers were designed to convert them into stable SCAR markers. Application of specific SCAR markers can be quickly detected and identified the corresponding mutant, to further verify the existence of variation. The test did not select the appropriate primers to distinguish “Jinxiu” and its mutant lines.