论文部分内容阅读
以盆栽考来木幼苗为试材,采用人工气候室模拟高温环境的方法,研究了高温干旱复合胁迫对考来木保护性酶系统生理活性的影响,以期为考来木的栽培与推广、园林植物的抗逆育种等提供科学理论依据,为植物生理生态研究贡献微薄的力量。结果表明:干旱或高温单一胁迫下,考来木幼苗的H_2O_2、O_2~·等活性氧代谢物含量及丙二醛(MDA)含量增加,超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)等抗氧化性酶活性亦同步显著增高。在高温、干旱复合胁迫下,活性氧代谢物含量及抗氧化性酶活性均显著高于单一胁迫,SOD活性近乎叠加增长;较之单一胁迫,复合胁迫下MDA含量并无显著升高。随胁迫时间延长或胁迫程度增加,抗氧化性酶活性持续增强;当胁迫增加到一定程度或持续一定时间后,抗氧化酶活性显著下降。干旱胁迫对考来木的伤害大于高温胁迫。结果表明,考来木可通过大幅提高抗氧化性酶系统的活性来抵御高温干旱胁迫所产生的活性氧伤害,因而具有较强的耐高温干旱能力。但抗氧化酶活性的提高是有限的,高强度或长时间胁迫下活性氧大量积累,抗氧化性酶被破坏而活性下降,则作物受害。
Taking potted seedlings as test materials, the artificial climate chamber was used to simulate the high temperature environment. The effects of high temperature and drought stress on the physiological activity of protective enzyme system of koalang were studied in order to promote the cultivation and popularization of koalai. Plant anti-retrograde breeding to provide a scientific theoretical basis for plant physiology and ecology contribute meager power. The results showed that under drought or high temperature single stress, the contents of H_2O_2, O_2 ~ · and other reactive oxygen species and the content of malondialdehyde (MDA) increased, while the activities of superoxide dismutase (SOD) and peroxidase POD), catalase (CAT) and other antioxidant enzyme activity simultaneously significantly increased. Under high temperature and drought stress, the content of active oxygen metabolites and the activities of antioxidant enzymes were significantly higher than those of single stress, and the activities of SOD increased almost in superposition. Compared with single stress, the content of MDA did not increase significantly under combined stress. With the prolonging of stress or the increase of stress, the activity of antioxidant enzyme continued to increase. When the stress increased to a certain extent or continued for a certain time, the activity of antioxidant enzyme decreased significantly. Drought stress on the Kao to wood injury than high temperature stress. The results showed that koalas could resist the damage of reactive oxygen species produced by high temperature and drought stress by greatly increasing the activity of antioxidant enzyme system and thus had a strong capability of resisting drought and drought. However, the increase of antioxidant enzyme activity is limited. Under high-intensity or prolonged stress, a large amount of active oxygen accumulates, the antioxidant enzyme is destroyed and the activity is decreased, and then the crop is damaged.