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采用冷浸法和溶剂萃取法,研究石菖蒲(Acorus gramineus Soland)根茎甲醇提取物对谷蠹Rhizopertha dominica(Fabricius)成虫的生物活性。结果表明,石菖蒲甲醇提取物以石油醚萃取物的生物活性最强,水层次之。石油醚萃取物对谷蠹具有较高的触杀、熏蒸、种群抑制活性和防治效果,并具有一定的驱避活性。药膜法触杀处理72h后对谷蠹的LC50和LC95分别为8.30μg/cm2,51.75μg/cm2。药纸熏蒸法6.25μL/L处理120h后对谷蠹的校正死亡率为94.25%。饲料拌药法250mg/kg处理对谷蠹的防效与5mg/kg马拉硫磷效果相当,且对F1代的种群抑制效果显著高于5mg/kg马拉硫磷处理。
Cold-immersed method and solvent extraction method were used to study the bioactivity of methanol extract of Rhizopertha dominica (Fabricius) from Acorus gramineus Soland. The results showed that petroleum ether extract of petroleum ether extract petroleum ether strongest biological activity, followed by water. Petroleum ether extract had higher contact toxicity, fumigation, population inhibitory activity and control effect, and had certain repellent activity. The LC50 and LC95 of T. gifuensis were 8.30μg / cm2,51.75μg / cm2 respectively after 72 hours exposure to the membrane method. The corrected fatality rate of Rhizoma alcedo in the paper fumigation 6.25 μL / L for 120 h was 94.25%. The control effect of feed mixed herbicide 250mg / kg on Dictyostellaria barbata was equivalent to that of 5mg / kg malathion, and the inhibitory effect on F1 population was significantly higher than that of malathion 5mg / kg.