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AIM:To investigate the differently expressed genes inhuman colorectal adenocarcinoma.METHODS:The integrated approach for geneexpression profiling that couples suppression subtractivehybridization,high-throughput cDNA array,sequencing,bioinformatics analysis,and reverse transcriptase real-time quantitative polymerase chain reaction(PCR)was carried out.A set of cDNA clones including 1260SSH inserts amplified by PCR was arrayed usingrobotic printing.The cDNA arrays were hybridizedwith florescent-labeled probes prepared from RNA ofhuman colorectal adenocarcinoma(HCRAC)and normalcolorectal tissues.RESULTS:A total of 86 genes were identified,16unknown genes and 70 known genes.The transcriptionfactor Sox9 influencing cell differentiation wasdownregulated.At the same time,Heat shock protein10 KDis downregulated and Calmoulin is up-regulated.CONCLUSION:Downregulation of heat shock protein 10KD lost its inhibition of Ras,and then attenuated the RasGTPase signaling pathway,increased cell proliferationand inhibited cell apoptosis.Down-regulated transcriptionfactor Sox9 influences cell differentiation and cell-specificgene expression.Down-regulated Sox9 also decreasesits binding to calmodulin,accumulates calmodulin asreceptor-activated kinase and phosphorylase kinase dueto the activation of PhK.
AIM: To investigate the differently expressed genes inhuman colorectal adenocarcinoma. METHODS: The integrated approach for gene expression profiling that couples suppression subtractive hybridization, high-throughput cDNA array, sequencing, bioinformatics analysis, and reverse transcriptase real-time quantitative polymerase chain reaction (PCR) was A set of cDNA clones including 1260 SSH inserts amplified by PCR was arrayed usingrobotic printing.The cDNA arrays were hybridizedwith florescent-labeled probes prepared from RNA ofhuman colorectal adenocarcinoma (HCRAC) and normalcolorectal tissues .RESULTS: A total of 86 genes were identified , 16unknown genes and 70 known genes. The transcription factor Sox9 influencing cell differentiation was reduced regulated. At the same time, Heat shock protein 10 KDis downregulated and Calmoulin is up-regulated. CONCLUSION: Downregulation of heat shock protein 10KD lost its inhibition of Ras, and then attenuated the RasGTPase signaling pathway, increased cell prolifer ation and inhibited cell apoptosis. Down-regulated transcription factor Sox9 contributing cell differentiation and cell-specific gene expression. Down-regulated Sox9 also decreases binding to calmodulin, accumulating calmodulin as receptor-activated kinase and phosphorylase kinase dueto the activation of PhK.