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慢性乙型肝炎(CHB)发生的重要原因之一是患者的机体不能对乙型肝炎病毒川BV)形成充分有效的免疫应答以清除病毒。树突状细胞(DC)是目前发现的功能最强的抗原呈递细胞,在激活T细胞介导的免疫中具有重要的作用。近期文献报道及我们的研究结果提示CHB患者DC不仅数量较少而且发育障碍、功能低下。所以促进CHB患者DC发育并改善其功能,对提高CHB患者的免疫功能以清除体内的HBV有重要意义。本文通过研究肝素在体外对CHB患者DC功能的调节作用,为寻找能促进DC成熟的经济有效的诱导因子提供实验依据。一、材料与方法 1．材料：巨噬细胞集落刺激因子(GM-CSF)(北京医科大学免疫室提供)；白细胞介素(IL)4、肿瘤坏死因子(TNF) α、干扰素(IFN)γ(美国Promega公司产品)；荧光标记的抗人CDla、CD40、CD80、CD83、CD86、人类白细胞抗原-DR 单克隆抗体(美国lmmunotech公司产品)；RPMI 1640培养基、MTT、异硫氰酸荧光素(FITC)-Dextran(美国Sigma
One of the important reasons for the development of chronic hepatitis B (CHB) is that the body of a patient can not form a sufficiently effective immune response to Hepatitis B virus (HBV) to clear the virus. Dendritic cells (DCs) are currently the most potent antigen-presenting cells found and play an important role in activating T cell-mediated immunity. Recently reported in the literature and our findings suggest that not only the number of DC in CHB patients is less and developmental disorders, dysfunction. So to promote the development of DC in CHB patients and improve their function, to improve the immune function of CHB patients to clear the body of HBV is of great significance. In this study, we investigated the regulatory effect of heparin on DC function in patients with CHB in vitro and provide experimental evidence for finding a cost-effective induction factor that can promote DC maturation. First, materials and methods 1. Materials: macrophage colony stimulating factor (GM-CSF) (provided by Beijing Medical University Immunization Room); interleukin (IL) 4; tumor necrosis factor alpha and interferon gamma (Promega, USA) ; Fluorescently labeled anti-human CDla, CD40, CD80, CD83, CD86, human leukocyte antigen-DR monoclonal antibody (product of lmmunotech, USA); RPMI 1640 medium, MTT, fluorescein isothiocyanate (FITC) American Sigma