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目的探讨海马神经元内钙离子浓度的动态变化在颞叶癫中的可能作用。方法应用新一代钙荧光指示剂Fluo3/AM,采用激光共聚焦扫描显微镜技术对颞叶癫大鼠海马神经元内的钙离子浓度变化进行动态观察。结果各时间点癫组大鼠海马神经细胞的平均荧光像素数(3h:634942±27735;12h:697066±14863;7d:732844±23107;60d:800030±16450)均明显高于对照组(3h:396499±31951;12h:389498±33257;7d:389809±29486;60d:392758±35197),差异有统计学意义(P<0.01);癫大鼠海马神经细胞内钙离子浓度的变化可分3个阶段:急性期细胞内的钙离子浓度先急剧升高,然后缓慢升高;静止期开始时钙离子浓度较急性期后期先稍有下降,然后再次缓慢上升;到慢性复发期,钙离子浓度达到最高峰,并维持在高水平。结论在颞叶癫的发生发展过程中海马细胞内存在钙稳态失调,该变化导致了神经元内游离钙离子浓度的急性和持久性升高,后者可能诱发了颞叶癫的自发性复发性发作。
Objective To investigate the possible role of dynamic changes of calcium concentration in hippocampal neurons in temporal lobe epilepsy. Methods Fluo3 / AM, a new generation of calcium fluorescence indicator, was used to observe the change of calcium concentration in hippocampal neurons of temporal lobe epileptic rats by laser confocal scanning microscopy. Results The average number of fluorescence pixels in the hippocampal neurons of the epileptic rats at each time point was significantly higher than that of the control group (3h: 634942 ± 27735; 12h: 697066 ± 14863; 7d: 732844 ± 23107; 60d: 800030 ± 16450) : 396499 ± 31951; 12h: 389498 ± 33257; 7d: 389809 ± 29486; 60d: 392758 ± 35197), the difference was statistically significant (P <0.01). The change of intracellular calcium concentration in hippocampal neurons of epileptic rats could be divided Three stages: the acute phase of intracellular calcium concentration increased rapidly, and then slowly increased; quiescent beginning of the calcium concentration than the first acute phase slightly decreased, and then slowly increased again; to chronic relapse, calcium Concentration reached its peak, and maintained at a high level. Conclusions There is calcium homeostasis in hippocampal cells during the development of temporal lobe epilepsy. This change leads to the acute and persistent increase of intracellular free calcium concentration in neurons, which may induce spontaneous development of temporal lobe epilepsy Recurrent seizures.