论文部分内容阅读
应用ＰＣＲ技术扩增并克隆恶性疟原虫海南、云南、安徽３个地理株ＭＳＡ１第二区基因，测定并分析其基因序列。结果表明，海南、云南、安徽株扩增片段长度为４２３ｂｐ，基因序列完全相同，我国虫株ＭＳＡ１与ＷＥＬＬＣＯＭＥ株最为相似，存在ＭＡＤ和Ｋ１两种等位基因型的基因内重组现象。
PCR technique was used to amplify and clone the second gene of MSA1 from three geographic strains of Plasmodium falciparum in Hainan, Yunnan and Anhui. The gene sequence was determined and analyzed. The results showed that the length of the amplified fragment was 423bp in Hainan, Yunnan and Anhui. The sequence of the amplified fragment was exactly the same. The MSA1 and WELLCOME strains in China were the most similar, with intragenic recombination of MAD and K1 alleles.