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目的：观察环孢菌素Ａ和吡喹酮联合应用对小鼠血吸虫性肝纤维化的影响。方法：感染第５ｗｋ末给药，吡喹酮８００ｍｇ／ｋｇ１次喂服及环孢菌素Ａ３０ｍｇ／ｋｇ皮下注射，每日１次，连续５日。结果：联合用药组血清游离羟脯氨酸水平在９、１２、１５ｗｋ分别为７．９１±０．２１、９．０３±１．６６、９．６９±１．２３μｍｏｌ／Ｌ，显著低于单独应用吡喹酮组的１０．１３±１．４５、１１．２３±１．３４和１１．８９±１．６２μｍｏｌ／Ｌ（Ｐ＜０．０１或Ｐ＜０．０５）；第９ｗｋ时肝脏虫卵肉芽肿面积（１．０８×１０５μｍ２）和其中胶原纤维的百分含量（１８．９１％±７．８２％），分别显著小于单独应用吡喹酮组（１．７２×１０５μｍ２和２９．４１％±１３．０９％）（Ｐ均＜０．０５）。结论：联合用药组的肝脏纤维化程度较单独用吡喹酮者为轻。
Objective: To observe the effect of combination of cyclosporin A and praziquantel on schistosomal hepatic fibrosis in mice. Methods: Infection at the end of the 5th wk administration, praziquantel 800mg / kg 1 times and cyclosporine A30mg / kg subcutaneous injection once daily for 5 days. Results: The level of serum free hydroxyproline in the combination group was 7.91 ± 0.21, 9.03 ± 1.66 and 9.69 ± 1.23μmol / L at 9, 12 and 15 weeks respectively, 10.13 ± 1.45, 11.23 ± 1.34 and 11.89 ± 1.62μmol / L (P <0.01 or P <0.05) of the praziquantel group, The area of egg granuloma (1.08 × 105μm2) and collagen fiber (18.91% ± 7.82%) were significantly smaller than that of praziquantel alone (1.72 × 105μm2 and 29.41 % ± 13.09%) (all P <0.05). Conclusion: The hepatic fibrosis in combination group is lighter than praziquantel alone.