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目的 观察哮喘豚鼠血清NO和IL -1浓度的变化。方法 2 4只豚鼠随机分为 3组 ,每组 8只。哮喘组 :豚鼠致敏用 10 %卵蛋白 1ml腹腔内注射 ,2周后用 1%卵蛋白吸入激发哮喘发作 ,每日 1次 ,连续 10d ;激素组 :在致敏豚鼠激发前每只豚鼠腹腔内注射 0 .5mg氢化可的松 ;对照组 :豚鼠致敏及激发均用生理盐水代替。测定各组豚鼠血清NO2 -/NO3-和IL -1浓度。结果 哮喘组血清NO2 -/NO3-和IL -1浓度较正常对照组明显增高 (P <0 .0 1) ,激素组血清NO2 -/NO3-和IL -1浓度较哮喘组明显降低 (P <0 .0 1)。结论 NO和IL -1在哮喘发病中起重要作用。
Objective To observe the changes of serum NO and IL - 1 levels in asthmatic guinea pigs. Methods 24 guinea pigs were randomly divided into 3 groups of 8 rats. Asthma group: guinea pig sensitized with 10% ovalbumin 1ml intraperitoneal injection, 2 weeks after asthma attack with 1% ovalbumin inhalation, once a day for 10 days; hormone group: before the sensitized guinea pigs peritoneal cavity 0.5mg hydrocortisone; control group: guinea pigs were sensitized and challenged with saline instead. The serum concentrations of NO2 - / NO3- and IL-1 in guinea pigs were measured. Results Serum levels of NO2 - / NO3- and IL - 1 in asthma group were significantly higher than those in normal control group (P <0.01), and levels of NO2 - / NO3- and IL - 1 in serum of asthma group were significantly lower than those in asthma group (P < 0 .0 1). Conclusion NO and IL-1 play an important role in the pathogenesis of asthma.