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[目的]初步了解山东东营胜利油田地区重点人群腹泻病原谱,获取该油区重点人群腹泻病流行病学数据,为临床用药和疾病监测提供参考依据。[方法]收集该油区腹泻粪便标本,提取标本中的细菌DNA,用基因芯片技术进行检测。芯片检测结果以PCR方法进行验证。用SPSS对腹泻感染率进行地区、年龄、性别因素分析。[结果]腹泻致病菌阳性24份,总阳性率为70.58%。检出腹泻致病菌8种,其中肠产毒性大肠杆菌(ETEC)阳性5份,阳性率14.70%;小肠结肠炎耶氏杆菌阳性4份,阳性率11.76%;空肠弯曲菌阳性4份,阳性率11.76%。各因素的统计学分析均无显著性差异。[结论]胜利油区腹泻病原谱分布较广,基地和外围感染率无显著差异。
[Objective] To understand the etiology spectrum of diarrhea in key population of Shengli Oilfield in Dongying, Shandong Province, get the epidemiological data of diarrhea in key population in this area, and provide reference for clinical medication and disease monitoring. [Method] The diarrhea stool samples of this area were collected, and the bacterial DNA in the samples was extracted and detected by gene chip technology. Chip test results to verify the PCR method. The incidence of diarrhea with SPSS regional, age, gender analysis. [Result] The positive rate of diarrheal pathogens was 24, the total positive rate was 70.58%. There were 8 pathogenic bacteria of diarrhea, including 5 enterotoxigenic Escherichia coli (ETEC) positive, the positive rate was 14.70%; 4 were Yersinia enterocolitica positive, the positive rate was 11.76%; 4 positive Campylobacter jejuni positive Rate of 11.76%. The statistical analysis of the factors showed no significant difference. [Conclusion] The distribution of pathogenic spectrum of diarrhea in Shengli Oil Region was broad, and there was no significant difference in infection rate between the base and the periphery.