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目的分析乙型肝炎患者外周血淋巴细胞亚群的表达。方法对112例乙型肝炎患者采用荧光定量PCR技术和流式细胞术分别检测血中HBVDNA及外周血淋巴细胞亚群CD3+T细胞、CD4+T细胞、CD8+T细胞、CD3-/CD16+CD56+(NK细胞)、CD3-CD19+(B细胞)表达百分比,进行各淋巴细胞亚群百分比统计学分析。结果与急性乙型肝炎(AHB)组比较,慢性乙型肝炎(CHB)组、肝炎肝硬化(LC)组CD3+T细胞、CD4+T细胞、CD8+T细胞、CD4+/CD8+比值均有显著性下降,LC组下降最显著(P<0.01)。而LC组B细胞百分比较CHB、AHB组高(P<0.01)。NK细胞百分比较CHB、AHB组均存在降低趋势(P<0.01)。结论乙型肝炎患者体内存在T细胞亚群失衡和细胞免疫功能紊乱;外周血T细胞亚群随病情进展而减少。
Objective To analyze the expression of peripheral blood lymphocyte subsets in patients with hepatitis B Methods One hundred and thirteen patients with hepatitis B were enrolled in this study. HBVDNA and peripheral blood lymphocyte subsets CD3 + T cells, CD4 + T cells, CD8 + T cells, CD3- / CD16 + cells were detected by fluorescence quantitative PCR and flow cytometry respectively. CD56 + (NK cells), CD3-CD19 + (B cells) expression percentage of each lymphocyte subsets statistical analysis. Results Compared with AHB group, the ratios of CD3 + T cells, CD4 + T cells, CD8 + T cells and CD4 + / CD8 + in chronic hepatitis B (CHB) group and cirrhosis group Sex decline, LC group decreased the most significant (P <0.01). The percentages of B cells in LC group were higher than those in CHB and AHB group (P <0.01). NK cell percentage than CHB, AHB group there is a decreasing trend (P <0.01). Conclusion Hepatitis B patients have imbalance of T cell subsets and cellular immune dysfunction. The T cell subsets of peripheral blood decrease with the progression of the disease.