论文部分内容阅读
目的：研究乳腺良恶性病变核仁组织区嗜银蛋白和细胞形态定量。方法：利用改良的ＡｇＮＯＲ 染色和传统的ＨＥ染色测定乳腺良恶性病变ＡｇＮＯＲ 颗粒面积、数目和细胞形态定量。结果：（１） 细胞面积、核面积、核质比值、细胞周长、ＡｇＮＯＲ 颗粒面积及颗粒数目在乳腺良恶性病变之间存在显著差别（ Ｐ＜ ０－０００５） ；（２） 细胞面积、核面积及颗粒面积在Ⅰ级乳腺癌与Ⅱ级和Ⅲ级乳腺癌之间有显著差别（ Ｐ＜ ０－０１） ，核质比值在Ⅰ级与Ⅲ级之间有显著差别（ Ｐ＜０－０１） ；（３） 颗粒数目在Ⅲ级乳腺癌与Ⅱ级、Ⅰ级乳腺癌有显著差别（Ｐ＜ ０－０５ 和Ｐ＜ ０－００５） ；（４） 细胞形态各参数与颗粒面积和颗粒数目高度相关。结论：ＡｇＮＯＲ 和细胞形态定量可作为乳腺良恶性病变鉴别、乳腺癌分级的客观指标。
Objective: To study the quantification of arginine-like protein and cell morphology in nucleolar regions of benign and malignant breast lesions. METHODS: AgNOR particle size, number and cell morphology were measured using modified AgNOR staining and conventional HE staining. Results: (1) There were significant differences in cell area, nuclear area, nuclear-to-cytoplasmic ratio, cell perimeter, AgNOR particle area, and number of particles between benign and malignant breast lesions (P < 0-0005); (2) Cell area and nucleus Area and particle area were significantly different between grade I breast cancer and grade II and grade III breast cancer (P<0-01), and the ratio of nuclear to cytoplasm was significantly different between grade I and grade III (P<0-01). (3) The number of particles in grade III breast cancer was significantly different from grade II, grade I breast cancer (P < 0-05 and P <0-005); (4) Cell morphology parameters and particle area and number of particles Highly correlated. Conclusion: AgNOR and cell morphology quantification can be used as the objective indicators for the differentiation of benign and malignant breast lesions and breast cancer grading.