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目的：探讨不同引物从杂交瘤细胞株中扩增出功能性和非功能性ＶＫ基因的关系。方法：利用ＲＴＰＣＲ技术扩增出轻链可变区基因，测序后，利用ＤＮＡＳＩＳ７和与ＧｅｎＢａｎｋ比较分析。结果：发现引物（ＶＬ５′．１和ＪＫ１／２）扩增出了非功能性ＶＫ基因。而改用引物（ＶＬ５′．２和ＶＬ３′）后，扩增出了有功能的ＶＫ基因。结论：非功能性ＶＫ基因的扩增，说明部分杂交瘤细胞出现了来源于原融合细胞的基因异常重排。在制备基因工程抗体中，区别功能性和非功能性ＶＫ基因具有重要意义。
Objective: To explore the relationship between functional and non-functional VK genes amplified from hybridoma cell lines by different primers. Methods: The light chain variable region gene was amplified by RTPCR technique. After sequencing, DNASIS7 was used to compare with GenBank. Results: Primers (VL5’.1 and JK1 / 2) were found to amplify non-functional VK genes. The use of primers (VL5’.2 and VL3 ’), amplified a functional VK gene. Conclusion: The amplification of non-functional VK gene indicates that some hybridoma cells have abnormal rearrangements of genes derived from the original fusion cells. In the preparation of genetically engineered antibodies, the distinction between functional and non-functional VK genes is of great importance.