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为获得５┐氟胞嘧啶（５┐ｆｌｕｏｒｏｃｙｔｏｓｉｎｅ，５┐ＦＣ）对含ＣＤ基因哺乳类细胞的“诱导”杀伤表型数据。方法小鼠成纤维细胞ＮＩＨ／３Ｔ３、大鼠神经胶质瘤细胞Ｃ６和包装细胞ＰＡ３１７，经基因修饰而表达非哺乳类酶－胞嘧啶脱氨酶（ｃｙ┐ｔｏｓｉｎｅｄｅａｍｉｎａｓｅ，ＣＤ）。结果体外，ＣＤ基因的表达几乎不影响细胞的生长速率。薄层层析分析（ＴＬＣ）表明，被含有重组逆转录病毒载体ｐＬＣＤＳＮ的假型病毒感染的哺乳类细胞及包装细胞的抽提液可将胞嘧啶代谢成脲嘧啶。５┐ＦＣ对有ＣＤ活性的细胞产生致死性细胞毒性。结论本实验支持了在５┐ＦＣ参与下，于肿瘤细胞内选择性表达ＣＤ的基因治疗方案。
To obtain the “inducible” killing phenotype data of 5 ┐fluorocytosine (5┐FC) for mammalian cells containing the CD gene. METHODS: Mouse fibroblasts NIH/3T3, rat glioma cells C6 and packaging cells PA317 were genetically modified to express non-mammalian enzymes, cy┐tosine deaminase (CD). Results In vitro, the expression of the CD gene hardly affected the cell growth rate. Thin layer chromatography analysis (TLC) showed that extracts of mammalian cells and packaging cells infected with a pseudotype virus containing recombinant retroviral vector pLCDSN can metabolize cytosine to uracil. 5 ┐FC produced lethal cytotoxicity to cells with CD activity. Conclusion This experiment supports the gene therapy protocol for selective expression of CD in tumor cells with the participation of 5┐FC.