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建立了一种超高效液相色谱-串联质谱分析芽苗菜中硫胺素、核黄素、吡哆醇、泛酸、生物素、叶酸、V_(B12)、烟酸、烟酰胺、Vc等10种水溶性维生素的方法。样品前处理用10%乙醇沉淀提取后,采用Waters Atalantis T_3色谱柱(2.1 mm×150 mm,3μm)分离,以0.1%甲酸-10.0%甲醇为流动相的梯度洗脱模式下,10种水溶性维生素在(10.0~1000.0)ng/m L浓度范围内线性良好,相关系数r2为0.9993~0.999,检出限范围为(0.10~11.5)μg/kg,定量限范围为(0.35~38.0)μg/kg,加标回收率为90.3%~99.5%,相对标准偏差(RSD)为2.0%~3.6%。该方法具有前处理简单、灵敏度高和检测速度快的优点,适用于芽苗菜中较宽浓度范围内多种维生素的同步分析,为其营养价值测定提供参考。
A method for the determination of thiamine, riboflavin, pyridoxine, pantothenic acid, biotin, folic acid, V_ (B12), niacin, nicotinamide and Vc in sprouts by ultra performance liquid chromatography-tandem mass spectrometry Water-soluble vitamins approach. The samples were pretreated with 10% ethanol and then separated on a Waters Atalantis T_3 column (2.1 mm × 150 mm, 3 μm). In the gradient elution mode with 0.1% formic acid-10.0% methanol as the mobile phase, 10 water- The calibration curve was linear over the range of (10.0-1000.0) ng / mL with a correlation coefficient r2 of 0.9993-0.999. The limit of detection ranged from (0.10 to 11.5) μg / kg and the limit of quantification ranged from 0.35 to 38.0 μg / kg, the spiked recoveries ranged from 90.3% to 99.5%, and the relative standard deviations (RSDs) ranged from 2.0% to 3.6%. The method has the advantages of simple pre-treatment, high sensitivity and fast detection speed, and is suitable for synchronous analysis of a plurality of vitamins in a wide range of concentration of sprout to provide reference for determining the nutritional value.