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痛行为实验表明，脑室注射胰高血糖素具有中枢抗阿片效应。本实验研究了胰高血糖素与吗啡和低频电针穴位刺激对大鼠脊髓背角神经元伤害性诱发放电的影响。结果显示：胰高血糖素预处理可有效对抗吗啡对大鼠脊髓背角神经元伤害性诱发放电的抑制效应；若在吗啡作用后１０ｍｉｎ与３０ｍｉｎ在脊髓背表面滴注胰高血糖素，亦可部分翻转吗啡的抑制效应。低频电针（１～３Ｈｚ）刺激大鼠同侧后肢＂足三里＂与＂三阴交＂穴位３０ｍｉｎ，可明显抑制大鼠脊髓背角神经元的放电活动；若以胰高血糖素作预处理，则低频电针的抑制效应明显减弱。单独注射胰高血糖素则可明显兴奋脊髓背角神经元的放电活动。以上结果提示：胰高血糖素在脊髓水平亦具有对抗吗啡和低频电针作用的效应；其抗阿片作用的机制可能通过脊髓内胰高血糖素受体与第二信使ｃＡＭＰ的作用来介导。
Pain behavior experiments show that intracerebroventricular injection of glucagon has a central anti-opiate effect. The present study was designed to investigate the effects of glucagon, morphine and low-frequency electroacupuncture (EAA) on the nociceptive firing in the dorsal horn of the spinal cord in rats. The results showed that glucagon preconditioning could effectively antagonize the inhibitory effect of morphine on the nociceptive discharge in the dorsal horn of spinal cord of rats. Glucagon may also be instilled into the spinal dorsal surface 10 min and 30 min after morphine administration Partial reversal of morphine inhibition. Low-frequency electroacupuncture (1 ~ 3Hz) stimulated ipsilateral hindlimb “Zusanli” and “Sanyinjiao” points for 30min in rats, which could significantly inhibit the discharge activity of spinal dorsal horn neurons in rats; if pretreatment with glucagon The inhibitory effect of low-frequency electroacupuncture was significantly weakened. Injecting glucagon alone significantly excites the firing activity of neurons in the dorsal horn of the spinal cord. The above results suggest that glucagon also has the effect of antagonizing the action of morphine and low frequency electro-acupuncture at spinal level. The mechanism of its anti-opioid effect may be mediated by the intracranial glucagon receptor and the second messenger cAMP.