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由于病毒只能在寄主活细胞内增殖,因而解决大量复制的技术问题,是“以病毒治虫”的首要环节。我们自1979年起开始研究赤松毛虫质型多角体病毒复制的有关技术,现将初步结果报道于下。一、病毒复制 1.在有松毛虫发生的松林,采集距结茧1-2个龄期的老幼虫,室内饲养在33×33×54厘米铁纱笼内,每笼300-500头,也可饲养于塑料薄膜围成的高50厘米的空间,大小视幼虫数量而定。一平方米可养虫2,000头,四周培砂压紧,上部敞开。在饲料松枝上用0.5亿/毫升多角体悬液均匀喷湿,松枝插入砂缽保
As the virus can only proliferate in the living cells of the host, the technical problem of coping with a large number of replication is the primary link of “virus-killing”. We have been studying the technology of replication of the polyhedrosis virus of Dendrolimus punctatus since 1979, and the preliminary results are reported below. First, the virus replication 1. In pine forest occurs pine caterpillar 1-2 moths collected from the cocoon, indoors feeding in 33 × 33 × 54 cm iron sarongs, 300-500 heads per cage, but also feeding Surrounded by plastic film 50 cm in space, depending on the size of larvae. 2,000 square meters per square meter can be raised, sand around the compaction, the upper part of the open. In the feed pine branches with 0.5 million / ml polyhedron suspension evenly wetting, pine branches into sand mortar